Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening
Identifieur interne : 000922 ( Main/Exploration ); précédent : 000921; suivant : 000923Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening
Auteurs : Sandra Duffy [Australie] ; Vicky M. Avery [Australie]Source :
- The American journal of tropical medicine and hygiene [ 0002-9637 ] ; 2012.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
With the increasing occurrence of drug resistance in the malaria parasite, Plasmodium falciparum, there is a great need for new and novel anti-malarial drugs. We have developed a 384-well, high-throughput imaging assay for the detection of new anti-malarial compounds, which was initially validated by screening a marine natural product library, and subsequently used to screen more than 3 million data points from a variety of compound sources. Founded on another fluorescence-based P. falciparum growth inhibition assay, the DNA-intercalating dye 4',6-diamidino-2-phenylindole, was used to monitor changes in parasite number. Fluorescent images were acquired on the PerkinElmer Opera High Throughput confocal imaging system and analyzed with a spot detection algorithm using the Acapella data processing software. Further optimization of this assay sought to increase throughput, assay stability, and compatibility with our high-throughput screening equipment platforms. The assay typically yielded Z'-factor values of 0.5-0.6, with signal-to-noise ratios of 12.
Affiliations:
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Le document en format XML
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Avery</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Discovery Biology, Griffith University</s1><s2>Nathan</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Nathan</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">12-0288826</idno><date when="2012">2012</date><idno type="stanalyst">PASCAL 12-0288826 INIST</idno><idno type="RBID">Pascal:12-0288826</idno><idno type="wicri:Area/PascalFrancis/Corpus">000087</idno><idno type="wicri:Area/PascalFrancis/Curation">000447</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000076</idno><idno type="wicri:doubleKey">0002-9637:2012:Duffy S:development:and:optimization</idno><idno type="wicri:Area/Main/Merge">000921</idno><idno type="wicri:Area/Main/Curation">000922</idno><idno type="wicri:Area/Main/Exploration">000922</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Development and Optimization of a Novel 384-Well Anti-Malarial Imaging Assay Validated for High-Throughput Screening</title><author><name sortKey="Duffy, Sandra" sort="Duffy, Sandra" uniqKey="Duffy S" first="Sandra" last="Duffy">Sandra Duffy</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Discovery Biology, Griffith University</s1><s2>Nathan</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Nathan</wicri:noRegion></affiliation></author><author><name sortKey="Avery, Vicky M" sort="Avery, Vicky M" uniqKey="Avery V" first="Vicky M." last="Avery">Vicky M. Avery</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Discovery Biology, Griffith University</s1><s2>Nathan</s2><s3>AUS</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Nathan</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">The American journal of tropical medicine and hygiene</title><title level="j" type="abbreviated">Am. j. trop. med. hyg.</title><idno type="ISSN">0002-9637</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">The American journal of tropical medicine and hygiene</title><title level="j" type="abbreviated">Am. j. trop. med. hyg.</title><idno type="ISSN">0002-9637</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antimalarial</term><term>Medical screening</term><term>Optimization</term><term>Parasiticide</term><term>Tropical medicine</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Optimisation</term><term>Antipaludique</term><term>Dépistage</term><term>Médecine tropicale</term><term>Antiparasitaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">With the increasing occurrence of drug resistance in the malaria parasite, Plasmodium falciparum, there is a great need for new and novel anti-malarial drugs. We have developed a 384-well, high-throughput imaging assay for the detection of new anti-malarial compounds, which was initially validated by screening a marine natural product library, and subsequently used to screen more than 3 million data points from a variety of compound sources. Founded on another fluorescence-based P. falciparum growth inhibition assay, the DNA-intercalating dye 4',6-diamidino-2-phenylindole, was used to monitor changes in parasite number. Fluorescent images were acquired on the PerkinElmer Opera High Throughput confocal imaging system and analyzed with a spot detection algorithm using the Acapella data processing software. Further optimization of this assay sought to increase throughput, assay stability, and compatibility with our high-throughput screening equipment platforms. The assay typically yielded Z'-factor values of 0.5-0.6, with signal-to-noise ratios of 12.</div></front></TEI><affiliations><list><country><li>Australie</li></country></list><tree><country name="Australie"><noRegion><name sortKey="Duffy, Sandra" sort="Duffy, Sandra" uniqKey="Duffy S" first="Sandra" last="Duffy">Sandra Duffy</name></noRegion><name sortKey="Avery, Vicky M" sort="Avery, Vicky M" uniqKey="Avery V" first="Vicky M." last="Avery">Vicky M. Avery</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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